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gmuG [2019-06-21 12:14:35]
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gmuG [2019-06-21 12:14:35]

beta-1,4-mannanase
locus
BSU05880
pI
5.63
mw
40.68 kDa
protein length
362 aa Sequence Blast
gene length
1089 bp Sequence Blast
function
glucomannan utilization
product
beta-1,4-mannanase
essential
no
ec
3.2.1.78
synonyms
ydhT
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.25|Utilization of glucomannan]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    632,774 633,862

    The protein

    Catalyzed reaction/ biological activity

  • Random hydrolysis of (14)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans (according to Swiss-Prot)

    • Protein family

  • glycosyl hydrolase 26 family (according to Swiss-Prot)

    • Structure

  • [PDB|2QHA], [http://www.proteopedia.org/wiki/index.php/3cbw 3CBW] mutant variant

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]-[gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]-[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]-[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]-[gene|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]-[gene|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE]-[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]-[gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]
    description
    [Pubmed|18177310]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18177310], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]: repression, [Pubmed|18177310], in [regulon|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|18177310], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • induced by cellobiose ([protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]) [Pubmed|18177310]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C195 (ydhT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2193 NBRP B. subtilis, Japan]
  • BKE05880 ([gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGCAACTCCCCCATTC, downstream forward: _UP4_TGAATCCGTAATGTCACAAT
  • BKK05880 ([gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGCAACTCCCCCATTC, downstream forward: _UP4_TGAATCCGTAATGTCACAAT

    • References

  • 19504356,18177310,18957862,19441796,20709850,20817675,24421132